High Fidelity (HiFi) Taq DNA Polymerase is an enzyme blend of recombinant Pfu and recombinant Taq DNA Polymerase with an efficient proofreading 3’ – 5’ exonuclease activity for improved PCR reactions. High Fidelity Taq DNA Polymerase is an efficient enzyme mixture that greatly increases fidelity and amplification of genomic targets up to 10 kb with high specificity, sensitivity, accuracy, and yield.
Advantages
Proofreading 3’ – 5’ exonuclease activity
High Fidelity
Recombinant sourced Taq DNA Polymerase
Format: 500 U
Amplification: genomic targets up to 10 kb
Storage: -20ºC for extended periods
Shipping: 2-8ºC (dry ice is not required)
3' – 5' Exonuclease Proofreading Ability: YES
5' – 3' Exonuclease Activity: YES
Applications
Full-length Gene Amplification, Gene Mutation Detection, L-PCR, PCR-ELISA
Source
HiFi Taq DNA Polymerase is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.
Composition
Storage Buffer: 20 mM Tris-HCl, pH8.8, 0.1 mM EDTA, 1 mM DTT, 1.0% Triton X-100, 50% Glycerol.
10X PCR Buffer: 100 mM KCl, 20 mM MgSO4·7H2O, 200 mM Tris-HCl, pH8.8, 1.0% Triton X-100, 100 mM (NH4)2SO4, 1 mg/ml BSA. KCl and (NH4)2SO4 are included in 10X PCR Buffer in order to both increase annealing temperature range and increase varying Mg2+ concentration tolerance.
Unit Definition
One unit catalyzes the incorporation of 10 nmol of dNTP into acid insoluble form in 30 minutes at 70ºC.
Quality Control
The quality of HiFi Taq DNA Polymerase is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. PCR is used to ensure consistent amplification of genomic targets using HiFi Taq DNA Polymerase.
Codice Prodotto: HT050
