The Presto™ Mini RNA Yeast Kit was designed for total RNA purification from yeast and a wide variety of other fungus species using an efficient RNA miniprep system. This RNA Kit includes Sorbitol Buffer to reduce sample preparation time and minimize hands on time. The Presto™ Mini RNA Yeast Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.
Advantages:
Purify total RNA within 20 minutes!
High Yield: 5-30 ?g of pure RNA
Convenient: Includes premixed Sorbitol Buffer
Sample: up to 5 x 107 yeast or other fungus species
RNA glass fiber spin column (yellow membrane optimized for total RNA purification from yeast)
Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
Storage: dry at room temperature (15-25°C) for up to 9 months
Applications:
RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis
Components:
Sorbitol Buffer
RB Buffer
W1 Buffer
Wash Buffer
RNase-free Water
RB Columns
2 ml Collection Tubes
Quality Control:
The quality of the Presto™ Mini RNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Codice Prodotto: RBY100
