GENEAID – PrestoMidi Plasmid DNA Purification Kit (Endotoxin Free)

    The Midi Plasmid Kit Endotoxin Free was designed for plasmid DNA purification and endotoxin removal using pre-packed anion-exchange resin midiprep columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells. This endotoxin free plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound by the plasmid midi column and the contaminants are removed with PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 2 hours without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun.