Prestained Protein Ladder (245 kDa)
Others/Protein
The Prestained Protein Ladder (245 kDa) is a three-color prestained protein standard which includes protein dye for convenient protein sample staining. The protein ladder consists of 12 prestained proteins covering a wide range of molecular weights (10 to 245 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in a gel loading buffer and is ready to use, without heating, diluting, or a reducing agent prior to loading.
DESCRIPTION
Advantages (Cat. # PL0245)
- Wide Molecular Weight range: 10-245 kDa
- Supplied buffer for direct gel loading
- Sharp bands with 28 and 75 kDa reference bands (green/red dye)
- Includes Protein Loading Dye (2 ml, 5X)
- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
- 1.5~2.5 μl per well for general Western transferring
- Increase application amount for thicker or larger gel
- Optional Dithiothreitol (DTT) procedure
STORAGE
- 25ºC for 2 weeks
- 4ºC for 3 months
- -20ºC for 24 months
APPLICATIONS
Monitor protein migration/sample in SDS-PAGE, Monitor protein transfer onto membranes during Western Blotting, Sizing of proteins on SDS-PAGE and Western blots
COMPONENTS
- Prestained Protein Ladder (approximately 0.1~0.4 mg/ml of each protein in buffer {20 mM Trisphosphate pH7.5 at 25°C, 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea and 15% (v/v) glycerol}.
- Protein Loading Dye (2 ml, 5X)
QUALITY CONTROL
The quality of the Prestained Protein Ladder (245 kDa) is tested on a lot-to-lot basis according to Geneaid's ISO certified quality management system. 5 μl of Prestained Protein Ladder (245 kDa) resolves 12 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocelluslose membrane.
